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recombinant mil-6  (PeproTech)


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    Structured Review

    PeproTech recombinant mil-6
    Recombinant Mil 6, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mil-6/mil+6/pmc10994929-254-48-52
    Average 90 stars, based on 1 article reviews
    recombinant mil-6 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    FACS:

    Article Title: Developmental self-reactivity determines pathogenic Tc17 differentiation potential of naive CD8 + T cells in murine models of inflammation
    Article Snippet: FACS-sorted CD44 lo CD5 lo Ly6C − , CD44 lo CD5 hi Ly6C − , and CD44 lo CD5 hi Ly6C + CD8 + T N cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorp TM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts.
    Article Snippet: Tumor-derived cytokines, such as interleukin (IL)-6, function in the context of tumor-to-host interactions, and their functions in immune-compromised hosts need to be addressed in the light of everincreasing number of patients under immunosuppression.. We studied the effects, in immune-comprised animals, of tumor-derived IL-6 on tumor growth using an experimental tumor vaccination model. Murine mammary carcinoma FM3A clone 25 (CL25) cells, which neither produce IL-6 nor express IL-6 receptors, were used. cDNA for murine IL-6 (mIL-6) was introduced to the CL25 cells, resulting in a high-producer (mIL-6H) clone.. In the severe combined immune-deficient (SCID) mice, the inoculation 3 weeks earlier of mIL-6H to a dorsal flank site suppressed the growth of the CL25 cells at the opposite flank site; a tumor-derived IL-6-mediated vaccination effect occurred.

    Article Title: Developmental self-reactivity determines the pathogenic Tc17 differentiation potential of naive CD8+ T cells by adjusting endogenous SMAD3 expression
    Article Snippet: FACS-sorted CD44loCD5loLy6C−, CD44loCD5hiLy6C−, and CD44loCD5hiLy6C+ CD8+ TN cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorpTM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts
    Article Snippet: In order to evaluate the effects of mIL-6 on the in vitro growth of CL25 cells, recombinant mIL-6 (PeproTech EC, Ltd., UK) was added on day 1 to the culture medium at 1,000, 100, 10, and 1 μg/l concentrations.

    Article Title: Regulation of Th17 cell differentiation and EAE induction by MAP3K NIK
    Article Snippet: The recombinant mIL-6 and hTGF-β were purchased from PeproTech (Rocky Hill, NJ), and recombinant LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) was from R&D Systems (Minneapolis, MN).

    Article Title: CRTAM controls residency of gut CD4 + CD8 + T cells in the steady state and maintenance of gut CD4 + Th17 during parasitic infection
    Article Snippet: 2 × 10 5 cells were activated with plate-bound anti-CD3 (10 μg/ml) and anti-CD28 (2 μg/ml) under polarizing TH1 conditions, i.e., recombinant IL-2 (PeproTech), mIL-12 (PeproTech), and anti–mouse IL-4 mAb (11B11), or TH17 conditions, i.e., recombinant mIL-6 (PeproTech), hTGFb, mIL-23, mAb anti-IL-4 (11B11), and anti–IFN-γ (RUGA2) for 5 d (primary stimulation).

    Article Title: NF-κB1 p105 regulates T-cell homeostasis and prevent chronic inflammation
    Article Snippet: The recombinant mIL-6 and hTGF-βwere purchased from PeproTech.

    Article Title: Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1
    Article Snippet: Recombinant mIL-6 (216-16), mTNF-α (315-01A), and mIL-1β (211-11B) were purchased from PeproTech (Rocky Hill, NJ, USA).

    Cell Culture:

    Article Title: Developmental self-reactivity determines pathogenic Tc17 differentiation potential of naive CD8 + T cells in murine models of inflammation
    Article Snippet: FACS-sorted CD44 lo CD5 lo Ly6C − , CD44 lo CD5 hi Ly6C − , and CD44 lo CD5 hi Ly6C + CD8 + T N cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorp TM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts.
    Article Snippet: Tumor-derived cytokines, such as interleukin (IL)-6, function in the context of tumor-to-host interactions, and their functions in immune-compromised hosts need to be addressed in the light of everincreasing number of patients under immunosuppression.. We studied the effects, in immune-comprised animals, of tumor-derived IL-6 on tumor growth using an experimental tumor vaccination model. Murine mammary carcinoma FM3A clone 25 (CL25) cells, which neither produce IL-6 nor express IL-6 receptors, were used. cDNA for murine IL-6 (mIL-6) was introduced to the CL25 cells, resulting in a high-producer (mIL-6H) clone.. In the severe combined immune-deficient (SCID) mice, the inoculation 3 weeks earlier of mIL-6H to a dorsal flank site suppressed the growth of the CL25 cells at the opposite flank site; a tumor-derived IL-6-mediated vaccination effect occurred.

    Article Title: Developmental self-reactivity determines the pathogenic Tc17 differentiation potential of naive CD8+ T cells by adjusting endogenous SMAD3 expression
    Article Snippet: FACS-sorted CD44loCD5loLy6C−, CD44loCD5hiLy6C−, and CD44loCD5hiLy6C+ CD8+ TN cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorpTM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts
    Article Snippet: In order to evaluate the effects of mIL-6 on the in vitro growth of CL25 cells, recombinant mIL-6 (PeproTech EC, Ltd., UK) was added on day 1 to the culture medium at 1,000, 100, 10, and 1 μg/l concentrations.

    Article Title: Regulation of Th17 cell differentiation and EAE induction by MAP3K NIK
    Article Snippet: The recombinant mIL-6 and hTGF-β were purchased from PeproTech (Rocky Hill, NJ), and recombinant LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) was from R&D Systems (Minneapolis, MN).

    Article Title: CRTAM controls residency of gut CD4 + CD8 + T cells in the steady state and maintenance of gut CD4 + Th17 during parasitic infection
    Article Snippet: 2 × 10 5 cells were activated with plate-bound anti-CD3 (10 μg/ml) and anti-CD28 (2 μg/ml) under polarizing TH1 conditions, i.e., recombinant IL-2 (PeproTech), mIL-12 (PeproTech), and anti–mouse IL-4 mAb (11B11), or TH17 conditions, i.e., recombinant mIL-6 (PeproTech), hTGFb, mIL-23, mAb anti-IL-4 (11B11), and anti–IFN-γ (RUGA2) for 5 d (primary stimulation).

    Article Title: NF-κB1 p105 regulates T-cell homeostasis and prevent chronic inflammation
    Article Snippet: The recombinant mIL-6 and hTGF-βwere purchased from PeproTech.

    Article Title: Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1
    Article Snippet: Recombinant mIL-6 (216-16), mTNF-α (315-01A), and mIL-1β (211-11B) were purchased from PeproTech (Rocky Hill, NJ, USA).

    Recombinant:

    Article Title: Developmental self-reactivity determines pathogenic Tc17 differentiation potential of naive CD8 + T cells in murine models of inflammation
    Article Snippet: FACS-sorted CD44 lo CD5 lo Ly6C − , CD44 lo CD5 hi Ly6C − , and CD44 lo CD5 hi Ly6C + CD8 + T N cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorp TM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts.
    Article Snippet: Tumor-derived cytokines, such as interleukin (IL)-6, function in the context of tumor-to-host interactions, and their functions in immune-compromised hosts need to be addressed in the light of everincreasing number of patients under immunosuppression.. We studied the effects, in immune-comprised animals, of tumor-derived IL-6 on tumor growth using an experimental tumor vaccination model. Murine mammary carcinoma FM3A clone 25 (CL25) cells, which neither produce IL-6 nor express IL-6 receptors, were used. cDNA for murine IL-6 (mIL-6) was introduced to the CL25 cells, resulting in a high-producer (mIL-6H) clone.. In the severe combined immune-deficient (SCID) mice, the inoculation 3 weeks earlier of mIL-6H to a dorsal flank site suppressed the growth of the CL25 cells at the opposite flank site; a tumor-derived IL-6-mediated vaccination effect occurred.

    Article Title: Developmental self-reactivity determines the pathogenic Tc17 differentiation potential of naive CD8+ T cells by adjusting endogenous SMAD3 expression
    Article Snippet: FACS-sorted CD44loCD5loLy6C−, CD44loCD5hiLy6C−, and CD44loCD5hiLy6C+ CD8+ TN cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorpTM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts
    Article Snippet: In order to evaluate the effects of mIL-6 on the in vitro growth of CL25 cells, recombinant mIL-6 (PeproTech EC, Ltd., UK) was added on day 1 to the culture medium at 1,000, 100, 10, and 1 μg/l concentrations.

    Article Title: Regulation of Th17 cell differentiation and EAE induction by MAP3K NIK
    Article Snippet: The recombinant mIL-6 and hTGF-β were purchased from PeproTech (Rocky Hill, NJ), and recombinant LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) was from R&D Systems (Minneapolis, MN).

    Article Title: CRTAM controls residency of gut CD4 + CD8 + T cells in the steady state and maintenance of gut CD4 + Th17 during parasitic infection
    Article Snippet: 2 × 10 5 cells were activated with plate-bound anti-CD3 (10 μg/ml) and anti-CD28 (2 μg/ml) under polarizing TH1 conditions, i.e., recombinant IL-2 (PeproTech), mIL-12 (PeproTech), and anti–mouse IL-4 mAb (11B11), or TH17 conditions, i.e., recombinant mIL-6 (PeproTech), hTGFb, mIL-23, mAb anti-IL-4 (11B11), and anti–IFN-γ (RUGA2) for 5 d (primary stimulation).

    Article Title: NF-κB1 p105 regulates T-cell homeostasis and prevent chronic inflammation
    Article Snippet: The recombinant mIL-6 and hTGF-βwere purchased from PeproTech.

    Article Title: Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1
    Article Snippet: Recombinant mIL-6 (216-16), mTNF-α (315-01A), and mIL-1β (211-11B) were purchased from PeproTech (Rocky Hill, NJ, USA).

    Expressing:

    Article Title: Developmental self-reactivity determines pathogenic Tc17 differentiation potential of naive CD8 + T cells in murine models of inflammation
    Article Snippet: FACS-sorted CD44 lo CD5 lo Ly6C − , CD44 lo CD5 hi Ly6C − , and CD44 lo CD5 hi Ly6C + CD8 + T N cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorp TM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts.
    Article Snippet: Tumor-derived cytokines, such as interleukin (IL)-6, function in the context of tumor-to-host interactions, and their functions in immune-compromised hosts need to be addressed in the light of everincreasing number of patients under immunosuppression.. We studied the effects, in immune-comprised animals, of tumor-derived IL-6 on tumor growth using an experimental tumor vaccination model. Murine mammary carcinoma FM3A clone 25 (CL25) cells, which neither produce IL-6 nor express IL-6 receptors, were used. cDNA for murine IL-6 (mIL-6) was introduced to the CL25 cells, resulting in a high-producer (mIL-6H) clone.. In the severe combined immune-deficient (SCID) mice, the inoculation 3 weeks earlier of mIL-6H to a dorsal flank site suppressed the growth of the CL25 cells at the opposite flank site; a tumor-derived IL-6-mediated vaccination effect occurred.

    Article Title: Developmental self-reactivity determines the pathogenic Tc17 differentiation potential of naive CD8+ T cells by adjusting endogenous SMAD3 expression
    Article Snippet: FACS-sorted CD44loCD5loLy6C−, CD44loCD5hiLy6C−, and CD44loCD5hiLy6C+ CD8+ TN cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorpTM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts
    Article Snippet: In order to evaluate the effects of mIL-6 on the in vitro growth of CL25 cells, recombinant mIL-6 (PeproTech EC, Ltd., UK) was added on day 1 to the culture medium at 1,000, 100, 10, and 1 μg/l concentrations.

    Article Title: Regulation of Th17 cell differentiation and EAE induction by MAP3K NIK
    Article Snippet: The recombinant mIL-6 and hTGF-β were purchased from PeproTech (Rocky Hill, NJ), and recombinant LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) was from R&D Systems (Minneapolis, MN).

    Article Title: CRTAM controls residency of gut CD4 + CD8 + T cells in the steady state and maintenance of gut CD4 + Th17 during parasitic infection
    Article Snippet: 2 × 10 5 cells were activated with plate-bound anti-CD3 (10 μg/ml) and anti-CD28 (2 μg/ml) under polarizing TH1 conditions, i.e., recombinant IL-2 (PeproTech), mIL-12 (PeproTech), and anti–mouse IL-4 mAb (11B11), or TH17 conditions, i.e., recombinant mIL-6 (PeproTech), hTGFb, mIL-23, mAb anti-IL-4 (11B11), and anti–IFN-γ (RUGA2) for 5 d (primary stimulation).

    Article Title: NF-κB1 p105 regulates T-cell homeostasis and prevent chronic inflammation
    Article Snippet: The recombinant mIL-6 and hTGF-βwere purchased from PeproTech.

    Article Title: Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1
    Article Snippet: Recombinant mIL-6 (216-16), mTNF-α (315-01A), and mIL-1β (211-11B) were purchased from PeproTech (Rocky Hill, NJ, USA).

    In Vitro:

    Article Title: Developmental self-reactivity determines pathogenic Tc17 differentiation potential of naive CD8 + T cells in murine models of inflammation
    Article Snippet: FACS-sorted CD44 lo CD5 lo Ly6C − , CD44 lo CD5 hi Ly6C − , and CD44 lo CD5 hi Ly6C + CD8 + T N cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorp TM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts.
    Article Snippet: Tumor-derived cytokines, such as interleukin (IL)-6, function in the context of tumor-to-host interactions, and their functions in immune-compromised hosts need to be addressed in the light of everincreasing number of patients under immunosuppression.. We studied the effects, in immune-comprised animals, of tumor-derived IL-6 on tumor growth using an experimental tumor vaccination model. Murine mammary carcinoma FM3A clone 25 (CL25) cells, which neither produce IL-6 nor express IL-6 receptors, were used. cDNA for murine IL-6 (mIL-6) was introduced to the CL25 cells, resulting in a high-producer (mIL-6H) clone.. In the severe combined immune-deficient (SCID) mice, the inoculation 3 weeks earlier of mIL-6H to a dorsal flank site suppressed the growth of the CL25 cells at the opposite flank site; a tumor-derived IL-6-mediated vaccination effect occurred.

    Article Title: Developmental self-reactivity determines the pathogenic Tc17 differentiation potential of naive CD8+ T cells by adjusting endogenous SMAD3 expression
    Article Snippet: FACS-sorted CD44loCD5loLy6C−, CD44loCD5hiLy6C−, and CD44loCD5hiLy6C+ CD8+ TN cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorpTM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts
    Article Snippet: In order to evaluate the effects of mIL-6 on the in vitro growth of CL25 cells, recombinant mIL-6 (PeproTech EC, Ltd., UK) was added on day 1 to the culture medium at 1,000, 100, 10, and 1 μg/l concentrations.

    Article Title: Regulation of Th17 cell differentiation and EAE induction by MAP3K NIK
    Article Snippet: The recombinant mIL-6 and hTGF-β were purchased from PeproTech (Rocky Hill, NJ), and recombinant LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) was from R&D Systems (Minneapolis, MN).

    Article Title: CRTAM controls residency of gut CD4 + CD8 + T cells in the steady state and maintenance of gut CD4 + Th17 during parasitic infection
    Article Snippet: 2 × 10 5 cells were activated with plate-bound anti-CD3 (10 μg/ml) and anti-CD28 (2 μg/ml) under polarizing TH1 conditions, i.e., recombinant IL-2 (PeproTech), mIL-12 (PeproTech), and anti–mouse IL-4 mAb (11B11), or TH17 conditions, i.e., recombinant mIL-6 (PeproTech), hTGFb, mIL-23, mAb anti-IL-4 (11B11), and anti–IFN-γ (RUGA2) for 5 d (primary stimulation).

    Article Title: NF-κB1 p105 regulates T-cell homeostasis and prevent chronic inflammation
    Article Snippet: The recombinant mIL-6 and hTGF-βwere purchased from PeproTech.

    Article Title: Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1
    Article Snippet: Recombinant mIL-6 (216-16), mTNF-α (315-01A), and mIL-1β (211-11B) were purchased from PeproTech (Rocky Hill, NJ, USA).

    Transfection:

    Article Title: Developmental self-reactivity determines pathogenic Tc17 differentiation potential of naive CD8 + T cells in murine models of inflammation
    Article Snippet: FACS-sorted CD44 lo CD5 lo Ly6C − , CD44 lo CD5 hi Ly6C − , and CD44 lo CD5 hi Ly6C + CD8 + T N cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorp TM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts.
    Article Snippet: Tumor-derived cytokines, such as interleukin (IL)-6, function in the context of tumor-to-host interactions, and their functions in immune-compromised hosts need to be addressed in the light of everincreasing number of patients under immunosuppression.. We studied the effects, in immune-comprised animals, of tumor-derived IL-6 on tumor growth using an experimental tumor vaccination model. Murine mammary carcinoma FM3A clone 25 (CL25) cells, which neither produce IL-6 nor express IL-6 receptors, were used. cDNA for murine IL-6 (mIL-6) was introduced to the CL25 cells, resulting in a high-producer (mIL-6H) clone.. In the severe combined immune-deficient (SCID) mice, the inoculation 3 weeks earlier of mIL-6H to a dorsal flank site suppressed the growth of the CL25 cells at the opposite flank site; a tumor-derived IL-6-mediated vaccination effect occurred.

    Article Title: Developmental self-reactivity determines the pathogenic Tc17 differentiation potential of naive CD8+ T cells by adjusting endogenous SMAD3 expression
    Article Snippet: FACS-sorted CD44loCD5loLy6C−, CD44loCD5hiLy6C−, and CD44loCD5hiLy6C+ CD8+ TN cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorpTM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts
    Article Snippet: In order to evaluate the effects of mIL-6 on the in vitro growth of CL25 cells, recombinant mIL-6 (PeproTech EC, Ltd., UK) was added on day 1 to the culture medium at 1,000, 100, 10, and 1 μg/l concentrations.

    Article Title: Regulation of Th17 cell differentiation and EAE induction by MAP3K NIK
    Article Snippet: The recombinant mIL-6 and hTGF-β were purchased from PeproTech (Rocky Hill, NJ), and recombinant LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) was from R&D Systems (Minneapolis, MN).

    Article Title: CRTAM controls residency of gut CD4 + CD8 + T cells in the steady state and maintenance of gut CD4 + Th17 during parasitic infection
    Article Snippet: 2 × 10 5 cells were activated with plate-bound anti-CD3 (10 μg/ml) and anti-CD28 (2 μg/ml) under polarizing TH1 conditions, i.e., recombinant IL-2 (PeproTech), mIL-12 (PeproTech), and anti–mouse IL-4 mAb (11B11), or TH17 conditions, i.e., recombinant mIL-6 (PeproTech), hTGFb, mIL-23, mAb anti-IL-4 (11B11), and anti–IFN-γ (RUGA2) for 5 d (primary stimulation).

    Article Title: NF-κB1 p105 regulates T-cell homeostasis and prevent chronic inflammation
    Article Snippet: The recombinant mIL-6 and hTGF-βwere purchased from PeproTech.

    Article Title: Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1
    Article Snippet: Recombinant mIL-6 (216-16), mTNF-α (315-01A), and mIL-1β (211-11B) were purchased from PeproTech (Rocky Hill, NJ, USA).

    Positive Control:

    Article Title: Developmental self-reactivity determines pathogenic Tc17 differentiation potential of naive CD8 + T cells in murine models of inflammation
    Article Snippet: FACS-sorted CD44 lo CD5 lo Ly6C − , CD44 lo CD5 hi Ly6C − , and CD44 lo CD5 hi Ly6C + CD8 + T N cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorp TM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts.
    Article Snippet: Tumor-derived cytokines, such as interleukin (IL)-6, function in the context of tumor-to-host interactions, and their functions in immune-compromised hosts need to be addressed in the light of everincreasing number of patients under immunosuppression.. We studied the effects, in immune-comprised animals, of tumor-derived IL-6 on tumor growth using an experimental tumor vaccination model. Murine mammary carcinoma FM3A clone 25 (CL25) cells, which neither produce IL-6 nor express IL-6 receptors, were used. cDNA for murine IL-6 (mIL-6) was introduced to the CL25 cells, resulting in a high-producer (mIL-6H) clone.. In the severe combined immune-deficient (SCID) mice, the inoculation 3 weeks earlier of mIL-6H to a dorsal flank site suppressed the growth of the CL25 cells at the opposite flank site; a tumor-derived IL-6-mediated vaccination effect occurred.

    Article Title: Developmental self-reactivity determines the pathogenic Tc17 differentiation potential of naive CD8+ T cells by adjusting endogenous SMAD3 expression
    Article Snippet: FACS-sorted CD44loCD5loLy6C−, CD44loCD5hiLy6C−, and CD44loCD5hiLy6C+ CD8+ TN cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorpTM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts
    Article Snippet: In order to evaluate the effects of mIL-6 on the in vitro growth of CL25 cells, recombinant mIL-6 (PeproTech EC, Ltd., UK) was added on day 1 to the culture medium at 1,000, 100, 10, and 1 μg/l concentrations.

    Article Title: Regulation of Th17 cell differentiation and EAE induction by MAP3K NIK
    Article Snippet: The recombinant mIL-6 and hTGF-β were purchased from PeproTech (Rocky Hill, NJ), and recombinant LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) was from R&D Systems (Minneapolis, MN).

    Article Title: CRTAM controls residency of gut CD4 + CD8 + T cells in the steady state and maintenance of gut CD4 + Th17 during parasitic infection
    Article Snippet: 2 × 10 5 cells were activated with plate-bound anti-CD3 (10 μg/ml) and anti-CD28 (2 μg/ml) under polarizing TH1 conditions, i.e., recombinant IL-2 (PeproTech), mIL-12 (PeproTech), and anti–mouse IL-4 mAb (11B11), or TH17 conditions, i.e., recombinant mIL-6 (PeproTech), hTGFb, mIL-23, mAb anti-IL-4 (11B11), and anti–IFN-γ (RUGA2) for 5 d (primary stimulation).

    Article Title: NF-κB1 p105 regulates T-cell homeostasis and prevent chronic inflammation
    Article Snippet: The recombinant mIL-6 and hTGF-βwere purchased from PeproTech.

    Article Title: Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1
    Article Snippet: Recombinant mIL-6 (216-16), mTNF-α (315-01A), and mIL-1β (211-11B) were purchased from PeproTech (Rocky Hill, NJ, USA).

    Flow Cytometry:

    Article Title: Developmental self-reactivity determines pathogenic Tc17 differentiation potential of naive CD8 + T cells in murine models of inflammation
    Article Snippet: FACS-sorted CD44 lo CD5 lo Ly6C − , CD44 lo CD5 hi Ly6C − , and CD44 lo CD5 hi Ly6C + CD8 + T N cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorp TM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts.
    Article Snippet: Tumor-derived cytokines, such as interleukin (IL)-6, function in the context of tumor-to-host interactions, and their functions in immune-compromised hosts need to be addressed in the light of everincreasing number of patients under immunosuppression.. We studied the effects, in immune-comprised animals, of tumor-derived IL-6 on tumor growth using an experimental tumor vaccination model. Murine mammary carcinoma FM3A clone 25 (CL25) cells, which neither produce IL-6 nor express IL-6 receptors, were used. cDNA for murine IL-6 (mIL-6) was introduced to the CL25 cells, resulting in a high-producer (mIL-6H) clone.. In the severe combined immune-deficient (SCID) mice, the inoculation 3 weeks earlier of mIL-6H to a dorsal flank site suppressed the growth of the CL25 cells at the opposite flank site; a tumor-derived IL-6-mediated vaccination effect occurred.

    Article Title: Developmental self-reactivity determines the pathogenic Tc17 differentiation potential of naive CD8+ T cells by adjusting endogenous SMAD3 expression
    Article Snippet: FACS-sorted CD44loCD5loLy6C−, CD44loCD5hiLy6C−, and CD44loCD5hiLy6C+ CD8+ TN cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorpTM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts
    Article Snippet: In order to evaluate the effects of mIL-6 on the in vitro growth of CL25 cells, recombinant mIL-6 (PeproTech EC, Ltd., UK) was added on day 1 to the culture medium at 1,000, 100, 10, and 1 μg/l concentrations.

    Article Title: Regulation of Th17 cell differentiation and EAE induction by MAP3K NIK
    Article Snippet: The recombinant mIL-6 and hTGF-β were purchased from PeproTech (Rocky Hill, NJ), and recombinant LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) was from R&D Systems (Minneapolis, MN).

    Article Title: CRTAM controls residency of gut CD4 + CD8 + T cells in the steady state and maintenance of gut CD4 + Th17 during parasitic infection
    Article Snippet: 2 × 10 5 cells were activated with plate-bound anti-CD3 (10 μg/ml) and anti-CD28 (2 μg/ml) under polarizing TH1 conditions, i.e., recombinant IL-2 (PeproTech), mIL-12 (PeproTech), and anti–mouse IL-4 mAb (11B11), or TH17 conditions, i.e., recombinant mIL-6 (PeproTech), hTGFb, mIL-23, mAb anti-IL-4 (11B11), and anti–IFN-γ (RUGA2) for 5 d (primary stimulation).

    Article Title: NF-κB1 p105 regulates T-cell homeostasis and prevent chronic inflammation
    Article Snippet: The recombinant mIL-6 and hTGF-βwere purchased from PeproTech.

    Article Title: Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1
    Article Snippet: Recombinant mIL-6 (216-16), mTNF-α (315-01A), and mIL-1β (211-11B) were purchased from PeproTech (Rocky Hill, NJ, USA).

    Clinical Proteomics:

    Article Title: Developmental self-reactivity determines pathogenic Tc17 differentiation potential of naive CD8 + T cells in murine models of inflammation
    Article Snippet: FACS-sorted CD44 lo CD5 lo Ly6C − , CD44 lo CD5 hi Ly6C − , and CD44 lo CD5 hi Ly6C + CD8 + T N cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorp TM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts.
    Article Snippet: Tumor-derived cytokines, such as interleukin (IL)-6, function in the context of tumor-to-host interactions, and their functions in immune-compromised hosts need to be addressed in the light of everincreasing number of patients under immunosuppression.. We studied the effects, in immune-comprised animals, of tumor-derived IL-6 on tumor growth using an experimental tumor vaccination model. Murine mammary carcinoma FM3A clone 25 (CL25) cells, which neither produce IL-6 nor express IL-6 receptors, were used. cDNA for murine IL-6 (mIL-6) was introduced to the CL25 cells, resulting in a high-producer (mIL-6H) clone.. In the severe combined immune-deficient (SCID) mice, the inoculation 3 weeks earlier of mIL-6H to a dorsal flank site suppressed the growth of the CL25 cells at the opposite flank site; a tumor-derived IL-6-mediated vaccination effect occurred.

    Article Title: Developmental self-reactivity determines the pathogenic Tc17 differentiation potential of naive CD8+ T cells by adjusting endogenous SMAD3 expression
    Article Snippet: FACS-sorted CD44loCD5loLy6C−, CD44loCD5hiLy6C−, and CD44loCD5hiLy6C+ CD8+ TN cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorpTM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts
    Article Snippet: In order to evaluate the effects of mIL-6 on the in vitro growth of CL25 cells, recombinant mIL-6 (PeproTech EC, Ltd., UK) was added on day 1 to the culture medium at 1,000, 100, 10, and 1 μg/l concentrations.

    Article Title: Regulation of Th17 cell differentiation and EAE induction by MAP3K NIK
    Article Snippet: The recombinant mIL-6 and hTGF-β were purchased from PeproTech (Rocky Hill, NJ), and recombinant LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) was from R&D Systems (Minneapolis, MN).

    Article Title: CRTAM controls residency of gut CD4 + CD8 + T cells in the steady state and maintenance of gut CD4 + Th17 during parasitic infection
    Article Snippet: 2 × 10 5 cells were activated with plate-bound anti-CD3 (10 μg/ml) and anti-CD28 (2 μg/ml) under polarizing TH1 conditions, i.e., recombinant IL-2 (PeproTech), mIL-12 (PeproTech), and anti–mouse IL-4 mAb (11B11), or TH17 conditions, i.e., recombinant mIL-6 (PeproTech), hTGFb, mIL-23, mAb anti-IL-4 (11B11), and anti–IFN-γ (RUGA2) for 5 d (primary stimulation).

    Article Title: NF-κB1 p105 regulates T-cell homeostasis and prevent chronic inflammation
    Article Snippet: The recombinant mIL-6 and hTGF-βwere purchased from PeproTech.

    Article Title: Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1
    Article Snippet: Recombinant mIL-6 (216-16), mTNF-α (315-01A), and mIL-1β (211-11B) were purchased from PeproTech (Rocky Hill, NJ, USA).

    Labeling:

    Article Title: Developmental self-reactivity determines pathogenic Tc17 differentiation potential of naive CD8 + T cells in murine models of inflammation
    Article Snippet: FACS-sorted CD44 lo CD5 lo Ly6C − , CD44 lo CD5 hi Ly6C − , and CD44 lo CD5 hi Ly6C + CD8 + T N cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorp TM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts.
    Article Snippet: Tumor-derived cytokines, such as interleukin (IL)-6, function in the context of tumor-to-host interactions, and their functions in immune-compromised hosts need to be addressed in the light of everincreasing number of patients under immunosuppression.. We studied the effects, in immune-comprised animals, of tumor-derived IL-6 on tumor growth using an experimental tumor vaccination model. Murine mammary carcinoma FM3A clone 25 (CL25) cells, which neither produce IL-6 nor express IL-6 receptors, were used. cDNA for murine IL-6 (mIL-6) was introduced to the CL25 cells, resulting in a high-producer (mIL-6H) clone.. In the severe combined immune-deficient (SCID) mice, the inoculation 3 weeks earlier of mIL-6H to a dorsal flank site suppressed the growth of the CL25 cells at the opposite flank site; a tumor-derived IL-6-mediated vaccination effect occurred.

    Article Title: Developmental self-reactivity determines the pathogenic Tc17 differentiation potential of naive CD8+ T cells by adjusting endogenous SMAD3 expression
    Article Snippet: FACS-sorted CD44loCD5loLy6C−, CD44loCD5hiLy6C−, and CD44loCD5hiLy6C+ CD8+ TN cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorpTM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts
    Article Snippet: In order to evaluate the effects of mIL-6 on the in vitro growth of CL25 cells, recombinant mIL-6 (PeproTech EC, Ltd., UK) was added on day 1 to the culture medium at 1,000, 100, 10, and 1 μg/l concentrations.

    Article Title: Regulation of Th17 cell differentiation and EAE induction by MAP3K NIK
    Article Snippet: The recombinant mIL-6 and hTGF-β were purchased from PeproTech (Rocky Hill, NJ), and recombinant LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) was from R&D Systems (Minneapolis, MN).

    Article Title: CRTAM controls residency of gut CD4 + CD8 + T cells in the steady state and maintenance of gut CD4 + Th17 during parasitic infection
    Article Snippet: 2 × 10 5 cells were activated with plate-bound anti-CD3 (10 μg/ml) and anti-CD28 (2 μg/ml) under polarizing TH1 conditions, i.e., recombinant IL-2 (PeproTech), mIL-12 (PeproTech), and anti–mouse IL-4 mAb (11B11), or TH17 conditions, i.e., recombinant mIL-6 (PeproTech), hTGFb, mIL-23, mAb anti-IL-4 (11B11), and anti–IFN-γ (RUGA2) for 5 d (primary stimulation).

    Article Title: NF-κB1 p105 regulates T-cell homeostasis and prevent chronic inflammation
    Article Snippet: The recombinant mIL-6 and hTGF-βwere purchased from PeproTech.

    Article Title: Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1
    Article Snippet: Recombinant mIL-6 (216-16), mTNF-α (315-01A), and mIL-1β (211-11B) were purchased from PeproTech (Rocky Hill, NJ, USA).

    Injection:

    Article Title: Developmental self-reactivity determines pathogenic Tc17 differentiation potential of naive CD8 + T cells in murine models of inflammation
    Article Snippet: FACS-sorted CD44 lo CD5 lo Ly6C − , CD44 lo CD5 hi Ly6C − , and CD44 lo CD5 hi Ly6C + CD8 + T N cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorp TM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts.
    Article Snippet: Tumor-derived cytokines, such as interleukin (IL)-6, function in the context of tumor-to-host interactions, and their functions in immune-compromised hosts need to be addressed in the light of everincreasing number of patients under immunosuppression.. We studied the effects, in immune-comprised animals, of tumor-derived IL-6 on tumor growth using an experimental tumor vaccination model. Murine mammary carcinoma FM3A clone 25 (CL25) cells, which neither produce IL-6 nor express IL-6 receptors, were used. cDNA for murine IL-6 (mIL-6) was introduced to the CL25 cells, resulting in a high-producer (mIL-6H) clone.. In the severe combined immune-deficient (SCID) mice, the inoculation 3 weeks earlier of mIL-6H to a dorsal flank site suppressed the growth of the CL25 cells at the opposite flank site; a tumor-derived IL-6-mediated vaccination effect occurred.

    Article Title: Developmental self-reactivity determines the pathogenic Tc17 differentiation potential of naive CD8+ T cells by adjusting endogenous SMAD3 expression
    Article Snippet: FACS-sorted CD44loCD5loLy6C−, CD44loCD5hiLy6C−, and CD44loCD5hiLy6C+ CD8+ TN cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorpTM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts
    Article Snippet: In order to evaluate the effects of mIL-6 on the in vitro growth of CL25 cells, recombinant mIL-6 (PeproTech EC, Ltd., UK) was added on day 1 to the culture medium at 1,000, 100, 10, and 1 μg/l concentrations.

    Article Title: Regulation of Th17 cell differentiation and EAE induction by MAP3K NIK
    Article Snippet: The recombinant mIL-6 and hTGF-β were purchased from PeproTech (Rocky Hill, NJ), and recombinant LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) was from R&D Systems (Minneapolis, MN).

    Article Title: CRTAM controls residency of gut CD4 + CD8 + T cells in the steady state and maintenance of gut CD4 + Th17 during parasitic infection
    Article Snippet: 2 × 10 5 cells were activated with plate-bound anti-CD3 (10 μg/ml) and anti-CD28 (2 μg/ml) under polarizing TH1 conditions, i.e., recombinant IL-2 (PeproTech), mIL-12 (PeproTech), and anti–mouse IL-4 mAb (11B11), or TH17 conditions, i.e., recombinant mIL-6 (PeproTech), hTGFb, mIL-23, mAb anti-IL-4 (11B11), and anti–IFN-γ (RUGA2) for 5 d (primary stimulation).

    Article Title: NF-κB1 p105 regulates T-cell homeostasis and prevent chronic inflammation
    Article Snippet: The recombinant mIL-6 and hTGF-βwere purchased from PeproTech.

    Article Title: Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1
    Article Snippet: Recombinant mIL-6 (216-16), mTNF-α (315-01A), and mIL-1β (211-11B) were purchased from PeproTech (Rocky Hill, NJ, USA).

    Control:

    Article Title: Developmental self-reactivity determines pathogenic Tc17 differentiation potential of naive CD8 + T cells in murine models of inflammation
    Article Snippet: FACS-sorted CD44 lo CD5 lo Ly6C − , CD44 lo CD5 hi Ly6C − , and CD44 lo CD5 hi Ly6C + CD8 + T N cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorp TM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts.
    Article Snippet: Tumor-derived cytokines, such as interleukin (IL)-6, function in the context of tumor-to-host interactions, and their functions in immune-compromised hosts need to be addressed in the light of everincreasing number of patients under immunosuppression.. We studied the effects, in immune-comprised animals, of tumor-derived IL-6 on tumor growth using an experimental tumor vaccination model. Murine mammary carcinoma FM3A clone 25 (CL25) cells, which neither produce IL-6 nor express IL-6 receptors, were used. cDNA for murine IL-6 (mIL-6) was introduced to the CL25 cells, resulting in a high-producer (mIL-6H) clone.. In the severe combined immune-deficient (SCID) mice, the inoculation 3 weeks earlier of mIL-6H to a dorsal flank site suppressed the growth of the CL25 cells at the opposite flank site; a tumor-derived IL-6-mediated vaccination effect occurred.

    Article Title: Developmental self-reactivity determines the pathogenic Tc17 differentiation potential of naive CD8+ T cells by adjusting endogenous SMAD3 expression
    Article Snippet: FACS-sorted CD44loCD5loLy6C−, CD44loCD5hiLy6C−, and CD44loCD5hiLy6C+ CD8+ TN cells were cultured in anti-CD3ε (2.5 μg/ml) (clone 145-2C11) and anti-CD28 (1 μg/ml) (clone 37.51) coated Nunc MaxiSorpTM Flat-Bottom Plate with recombinant mIL-6 (20 ng/ml) (PeproTech), rmTGF-β (2.5 ng/ml) (PeproTech), anti-IFN-γ (10 μg/ml) (clone XMG1.2), and anti-IL-4 (10 μg/ml) (clone 11B11) for 3 days.

    Article Title: Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts
    Article Snippet: In order to evaluate the effects of mIL-6 on the in vitro growth of CL25 cells, recombinant mIL-6 (PeproTech EC, Ltd., UK) was added on day 1 to the culture medium at 1,000, 100, 10, and 1 μg/l concentrations.

    Article Title: Regulation of Th17 cell differentiation and EAE induction by MAP3K NIK
    Article Snippet: The recombinant mIL-6 and hTGF-β were purchased from PeproTech (Rocky Hill, NJ), and recombinant LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) was from R&D Systems (Minneapolis, MN).

    Article Title: CRTAM controls residency of gut CD4 + CD8 + T cells in the steady state and maintenance of gut CD4 + Th17 during parasitic infection
    Article Snippet: 2 × 10 5 cells were activated with plate-bound anti-CD3 (10 μg/ml) and anti-CD28 (2 μg/ml) under polarizing TH1 conditions, i.e., recombinant IL-2 (PeproTech), mIL-12 (PeproTech), and anti–mouse IL-4 mAb (11B11), or TH17 conditions, i.e., recombinant mIL-6 (PeproTech), hTGFb, mIL-23, mAb anti-IL-4 (11B11), and anti–IFN-γ (RUGA2) for 5 d (primary stimulation).

    Article Title: NF-κB1 p105 regulates T-cell homeostasis and prevent chronic inflammation
    Article Snippet: The recombinant mIL-6 and hTGF-βwere purchased from PeproTech.

    Article Title: Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1
    Article Snippet: Recombinant mIL-6 (216-16), mTNF-α (315-01A), and mIL-1β (211-11B) were purchased from PeproTech (Rocky Hill, NJ, USA).



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